Tet3 antibody (pAb)

RRID: AB_2793618
Catalog No: 61395 Format: 100 µl $470 Buy
Catalog No: 61396 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
200 kDa
Reactivity:
Mouse

Applications

ChIP Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:1,000 - 1:5,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 1159-1329 of mouse Tet3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

 

Tet3 antibody (pAb) tested by Western blot.
The blot was probed with Tet3 antibody at a dilution of 1:5,000.

Lane 1: 293T cells transfected with Tet3.
Lane 2: Mouse lung.
Lane 3: Mouse muscle.
Lane 4: Mouse ovary.
Lane 5: Mouse spleen.
Lane 6: Mouse testes.
Lane 7: Mouse thymus.
Lane 8: 293T cells transfected with Tet1.
Lane 9: 293T cells transfected with Tet2.

Background

Tet3 (tet methylcytosine dioxygenase 3) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Tet3 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting