RBBP5 antibody (pAb)

Aliases: retinoblastoma binding protein 5, RBQ3, SWD1
Catalog No: 61405 Format: 100 µl $445 Buy
Catalog No: 61406 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
70 kDa
Reactivity:
Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 - 1:5,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Immunogen

This antibody was raised against a peptide within the C-terminal region of human RBBP5.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
RBBP5 antibody (pAb) tested by ChIP-Seq.

RBBP5 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of human PBMC chromatin and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 21 million sequence tags were mapped to identify RBBP5 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

RBBP5 antibody (pAb) tested by Western blot.

RBBP5 antibody (pAb) tested by Western blot.
MCF-7 whole-cell extract (20 µg) probed with RBBP5 antibody at a dilution of 1:1,000.

Background

As part of the MLL1/MLL complex, RBBP5 (retinoblastoma binding protein 5) is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.

Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for RBBP5 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RBBP5 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting