WDR5 antibody (pAb)

Aliases: SWD3, BIG-3
Catalog No: 61485 Format: 100 µl $435 Buy
Catalog No: 61486 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
40 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide within the C-terminal region of human WDR5.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
WDR5 antibody (pAb) tested by Western blot.

WDR5 antibody (pAb) tested by Western blot.
Nuclear extract (30 µg) of Raji (Lane 1) and HeLa cells (Lane 2) probed with WDR5 antibody (pAb) at a 1:500 dilution.

Background

WDR5 (WD repeat-containing protein 5) is a component of the Set1 histone methyltransferase / MLL1/MLL complex that is involved in methylation and dimethylation of 'Lys-4' of Histone H3. H3K4 methylation represents a specific tag for epigenetic transcriptional activation. WDR5 is also a component of the NSL complex and it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. WDR5 is also core component of other methyltransferase-containing complexes including MLL2/3 (also named ASCOM complex) and MLL4/WBP7.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting