SMARCC1 / BAF155 antibody (pAb)

Aliases: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 / BRG1-associated factor 155, Rsc8, SWI3, BAF155, CRACC1
Catalog No: 61409 Format: 100 µl $435 Buy
Catalog No: 61410 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
155 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide within the N-terminal region of human SMARCC1 / BAF155.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
SMARCC1 / BAF155 antibody (pAb) tested by immunofluorescence.

SMARCC1 / BAF155 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCC1 / BAF155 antibody at a 1:2,000 dilution.

SMARCC1 / BAF155 antibody (pAb) tested by Western blot.

SMARCC1 / BAF155 antibody (pAb) tested by Western blot.
K-562 nuclear extract (30 µg) probed with SMARCC1 / BAF155 antibody at a dilution of 1:500.

Background

SMARCC1 / BAF155 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 / BRG1-associated factor 155) is involved in transcriptional activation and repression of select genes by chromatin remodeling. Component of multiple SWI/SNF chromatin remodeling complexes including Swi/Snf-A (BAF), Swi/Snf-B (PBAF), Brm, Brg1(I), WINAC, Brg1(II), npBAF and nBAF.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for SMARCC1 / BAF155 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCC1 / BAF155 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting