DNMT1 monomethyl Lys142 antibody (pAb)

RRID: AB_2793659
Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
Catalog No: 61497 Format: 100 µl $460 Buy
Catalog No: 61498 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
200 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

For DNMT1, we also offer AbFlex® DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a peptide including monomethyl lysine 142 of human DNMT1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 µg) probed with DNMT1 monomethyl Lys142 antibody (1:500).

DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Monomethyl Lys142 peptide.
    Lane 2: Dimethyl lysine 142 peptide.
    Lane 3: Trimethyl lysine142 peptide.
    Lane 4: Unmodified lysine 142 peptide.

Background

DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Methylation at lysine 142 by SETD7 promotes DNMT1 proteasomal degradation while phosphorylation of serine 143 via AKT blocks degradation. These modifications appear to play an important role in regulating DNMT enzyme activity and thus levels of DNA methylation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting