CXXC4 antibody (pAb)

RRID: AB_2793732
Aliases: Idax, 9330210J02Rik, C030003J12Rik
Catalog No: 61679 Format: 100 µl $460 Buy
Catalog No: 61680 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
42 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:1,000 - 1:2,500 dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This antibody was raised against a peptide within the N-terminal region of mouse Cxxc4.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Cxxc4 antibody (pAb) tested by Western blot.
Detection of Cxxc4 antibody by Western blot analysis.
Lane 1: Mouse ES cytoplasmic extract.
Lane 2: F9 cytoplasmic extract.
Lane 3: P19 cytoplasmic extract.
Lane 4: HEK293 whole cell extract.
30 µg of lysate per lane probed with Cxxc4 antibody at a dilution of 1:1,000.

Background

Cxxc4 (CXXC Finger Protein 4) acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1 and has been implicated in malignant renal cell carcinoma and colonic villous adenoma. Recently, Cxxc4 has been shown to be involved in the regulation of DNA demethylation. It binds to unmethylated CpGs in DNA and is involved in TET2 downregulation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting