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Recombinant NSUN5 protein
| Catalog No: 81173 | Format: 20 µg | $450 | Add to Cart |
| Catalog No: 81873 | Format: 1 mg | $4,050 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
NSUN5 (NOP2/Sun Domain Family, Member 5), also called as NOL1R, is a member of NSUN family. It is a S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 in 28S rRNA.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Protein Details
Recombinant NSUN5 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_683759.1) with an N-terminal FLAG tag. The molecular weight of the protein is 52.1 kDa.
Recombinant NSUN5 protein
10% SDS-PAGE Coomassie staining
MW: 52.1 kDa
Purity: >85%
MTase-Glo assay for NSUN5 methyltransferase activity
400 ng total RNA and 1 μM SAM was incubated with different concentrations of NSUN5 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/μL RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.
MTase-Glo assay for NSUN5 methyltransferase activity
400 ng total RNA and 1 μM SAM was incubated with different concentrations of NSUN5 protein in 8 μl reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/μL RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
