Recombinant DDX54 protein

Aliases: ATP-dependent RNA helicase DDX54, DP97, DEAD-Box Helicase 54
Catalog No: 82144 Format: 20 µg $505 Add to Cart
Catalog No: 82744 Format: 1 mg $4,500 Add to Cart

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

DDX54 (ATP-dependent RNA helicase DDX54) is a nucleolar DEAD-box RNA helicase that functions as a key regulator of transcription and ribosome biogenesis. It is recruited to gene promoters, including those of nuclear receptors and stress-responsive genes, where it acts as a transcriptional co-regulator to modulate their expression. Furthermore, DDX54 plays a direct role in the nucleolar stages of ribosomal RNA (rRNA) processing and maturation, which is essential for the proper assembly of ribosomes. Its activity is also implicated in the cellular stress response, where it contributes to the reorganization of nucleolar architecture and halts rRNA synthesis to conserve energy. Dysregulation of DDX54 has been associated with cancers, underscoring its importance in fundamental cell growth and proliferation pathways.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant DDX54 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001104792.1) with an N-terminal FLAG tag. The molecular weight of DDX54 is 99.94 kDa.

References

Human

 
DDX54 Coomassie gel

Recombinant DDX54 protein gel.
DDX54 protein was run on an 4-20% SDS-PAGE gel and stained with Coomassie blue.

MW: 99.94 kDa

Purity: > 90%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.