Recombinant NFATC1 protein

Aliases: Nuclear factor activated T-cells, cytoplasmic 1
Catalog No: 82148 Format: 20 µg $470 Add to Cart
Catalog No: 82748 Format: 1 mg $4,150 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant NFATC1 protein gel

Recombinant NFATC1 protein gel
4-20% SDS-PAGE gel
MW: 102.51 kDa
Purity: >95%

HTRF Assay for NFATC1 activity.

HTRF Assay for Recombinant NFATC1 activity.
1 µM substrate dsDNA (5’ biotin-CTCAATGGAAAATGC 3’) was incubated with different concentrations of NFATC1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour . Anti-FLAG antibody was used to detect reaction products.

Background

NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1) is a transcription factor that plays a pivotal role in the immune system, particularly in T-cell and B-cell activation, development, and differentiation. It resides in the cytoplasm in an inactive, phosphorylated state. Upon cell stimulation, such as through T-cell receptor engagement, a calcium-dependent calcineurin signal triggers its dephosphorylation, leading to its rapid translocation into the nucleus. Inside the nucleus, NFATC1 binds to specific DNA sequences and, in cooperation with other transcription factors like AP-1, orchestrates the expression of genes critical for the immune response, including those encoding cytokines and cell surface receptors. Beyond immunology, it is also essential in other biological processes such as cardiac valve development, osteoclast genesis (bone remodeling), and adipocyte differentiation. Its dysregulation is associated with autoimmune diseases and cancer.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting