Recombinant PARP8 protein

Aliases: poly (ADP-ribose) polymerase
Catalog No: 82163 Format: 20 µg $480 Add to Cart
Catalog No: 82763 Format: 1 mg $4,150 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant PARP8 protein gel

Recombinant PARP8 protein gel
4-20% SDS-PAGE gel
MW: 97.14 kDa
Purity: >90%

HTRF Assay for PARP8 activity.

ELISA for PARP8 activity
25 μM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP8 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter plate reader.

Background

PARP8 is an atypical member of the poly(ADP-ribose) polymerase (PARP) family with mono-ADPribosyltransferase (MARylase) activity. Unlike canonical PARPs involved in DNA repair, PARP8 localizes to the nuclear envelope and modifies specific target proteins, playing a role in nuclear pore complex biology, cellular stress response, and potentially in regulating gene expression. Its precise physiological functions and involvement in disease are still under active investigation, but it represents a distinct regulatory component within the broader PARP-mediated signaling network.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting