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Recombinant PARP7 protein
| Aliases: Protein mono-ADP-ribosyltransferase TIPARP, TiPARP, ARTD14, DDF1, RM1, PART14 |
| Catalog No: 82149 | Format: 10 µg | $415 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PARP7(Protein mono-ADP-ribosyltransferase TIPARP), also known as TiPARP, is a mono ADPribosyltransferase belonging to the Poly-ADP-ribose polymerase (PARP) family. Unlike some PARPs involved in DNA repair, PARP7’s primary functions are linked to regulating gene expression and the immune response, particularly the type I interferon signaling pathway. It is induced by the transcription factor AHR (aryl hydrocarbon receptor) and acts as a negative feedback regulator. PARP7 modifies itself and other target proteins with a single ADP-ribose unit (MARylation), which influences their activity and stability. By MARylating key transcription factors like AHR and IRF3/7, it dampens inflammatory and interferon responses, thereby maintaining immune homeostasis. Its role as a checkpoint in anti-tumor immunity has made it a emerging target for cancer immunotherapy, where its inhibition is sought to enhance the body’s innate immune attack against cancer cells.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant PARP7 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_056323.2) with an N-terminal FLAG tag. The molecular weight of PARP7 is 77.5 kDa.
References
Human
Recombinant PARP7 protein gel.
PARP7 protein was run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue.
MW: 77.5 kDa
Purity: >80%
ELISA for PARP7 activity
25 μM NAD-Biotin and PARP7 were added into ELISA plate (coated with histone H2A and H2B mixture) in ADPR buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
