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Recombinant PARP-3 protein
| Aliases: Protein mono ADP ribosyltransferase PARP3, ADPRT3, ARTD3, IRT1 |
| Catalog No: 82027 | Format: 20 µg | $505 | Add to Cart |
| Catalog No: 82627 | Format: 1 mg | $4,150 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PARP3 (Protein mono-ADP-ribosyltransferase PARP3), also known as ADPRT3, ADPRTL2, ADPRTL3, ARTD3, IRT1 and PADPRT-3. PARP3 is a Mono-ADP-ribosyltransferase that mediates mono-ADP ribosylation of target proteins and plays a key role in the response to DNA damage. Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins. Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6. Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on ‘Glu-2’ (H2BE2ADPr) of nucleosomes containing nicked DNA.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant PARP3 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_005476.4) with an N-terminal His and FLAG tag. The molecular weight of the protein is 64.6 kDa.
References
Human
Recombinant PARP3 protein gel
10% SDS-PAGE Comassie staining
MW: 64.6 kDa
Purity: >95%
ELISA for PARP3 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP3 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of arrival.
