AbFlex® MAZ antibody (rAb)

Aliases: MYC Associated Zinc Finger Protein, Transcription Factor Zif87, ZNF801
Catalog No: 91205 Format: 100 µg $385 Buy
Catalog No: 91206 Format: 10 µg $80 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
55 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 4 µg/ml dilution.

Note: For optimal results, primary antibody incubations should be performed
at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions
may also reduce background. Individual optimization may be required.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39935.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against human Maz protein [MYC-associated zinc finger protein], NP_002374.

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

References

 
AbFlex<sup>®</sup> MAZ antibody (rAb) tested by Western blot.

AbFlex® MAZ antibody (rAb) tested by Western Blot.
10 µg MCF7 cell nuclear extract was run on SDS-PAGE, and probed with 2 µg/ml AbFlex MAZ antibody. MW: 55 kDa

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.  MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting