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AbFlex® SF3B1 antibody (rAb)
| Aliases: Splicing factor 3b subunit 1, MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155 |
| Catalog No: 91525 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91526 | Format: 10 µg | $90 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.2 - 1 µg/mL
IP: 2 µg per IP
IF: 0.4 - 2 µg/mL
Immunogen
This SF3B1 antibody was raised against a peptide of human SF3B1.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® SF3B1 rAb antibody tested by Western blot.
40 µg of HepG2 (Lane1) and HeLa (Lane 2) whole cell extracts were probed with AbFlex® SF3B1 antibody at 0.5 µg/mL.
AbFlex® SF3B1 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate SF3B1 from 1 mg of HepG2 whole cell extract, 40% of IP product (Lane 3) and 40 µg of Input proteins (Lane 1) was run on an SDS-PAGE and probed with the same antibody at 0.2 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as negative control for IP (Lane 2).
AbFlex® SF3B1 rAb antibody tested by immunofluorescence.
SF3B1 was detected in human HeLa cells using AbFlex SF3B1 (rAb) at 2 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat AntiRabbit Recombinant Secondary Antibody (H+L).
AbFlex® SF3B1 rAb antibody tested by Western blot on mouse tissue
40 µg of mouse extracts were probed with AbFlex® SF3B1 antibody at 0.2 µg/mL.
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® CCNB1 (Cyclin B1) antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
AbFlex® SF3B1 antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalianHEK293 cells.
SF3B1 (splicing factor 3b subunit 1, also known as MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155) is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
