Aurora B antibody (pAb)

RRID: AB_2793209
Aliases: Aurora Kinase B, AurB, ARK2
Catalog No: 39261 Format: 200 µl $460 Buy
Catalog No: 39262 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
49 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:200 - 1:1,000 dilution
WB: 1:500 - 1:5,000 dilution

Immunogen

This Aurora B antibody was raised against a peptide including the C-terminus of human Aurora B.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Aurora B pAb tested by immunofluorescence.
Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

Aurora B pAb tested by Western blot
Nuclear extract of U2OS cells (20 µg per lane) probed with Aurora B polyclonal antibody (1:500 dilution).

Background

Aurora B (Aurora Kinase B, AurB, ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis. Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase. Aurora B phosphorylates a number of substrates, including INCENP, CENP-A, and Histone H3. Misregulation of Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Aurora B antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting