AbFlex® HP1β antibody (rAb)

Aliases: CBX, M31, MOD1, p25beta, HP1Hsbeta, HP1Hs-beta
Catalog No: 91407 Format: 100 µg $350 Buy
Catalog No: 91408 Format: 10 µg $75 Buy

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Antibody Type:
Recombinant
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
25 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Enzyme-linked Immunosorbent Assay Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.5-2 µg/ml dilution

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental antibody clone, see Catalog No. 39979.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications: IP ICC/IF WB ELISA View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HP1β antibody was raised against recombinant mouse HP1β.

Buffer

Purified in 140 mM Hepes (pH 7.5), 70 mM NaCl, 32 mM NaOAc, 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.

 

AbFlex® HP1 beta antibody (rAb) tested by Western blot.
20 µg of HeLa whole cell extract was run on SDS-PAGE and probed with AbFlex HP1 beta antibody at 0.5 µg/ml.

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex®HP1 beta antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

HP1 beta (Heterochromatin Protein 1 beta, Chromobox protein 1, CBX1) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 beta contains a chromo domain and a chromo shadow domain. Through the chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Interaction between HP1 beta and the lamin B receptor can facilitate the sequestration of heterochromatin to the nuclear envelope.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting