AATF / Che-1 antibody (mAb)

Clone: 1B2D8
Catalog No: 61789 Format: 100 µg $435 Buy
Catalog No: 61790 Format: 10 µg $105 Buy

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Antibody Type:
Protein A Chromatography
Human, Mouse


Western Blot Validated Immunofluorescence Validated

Application Notes

Validated Applications:
WB: 2 - 4 µg/ml
IF: 1:100 - 1:500

Published Applications:

Ishigaki et. al., 2010 Cell Death Differ. 17: 774-786

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against peptide comprising amino acids 1-39 of mouse AATF (Apoptosis-antagonizing transcription factor) (NP_062790).


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. 

AATF / Che-1 antibody (mAb) tested by immunofluorescence.

AATF / Che-1 antibody (mAb) tested by immunofluorescence.
Left: Formaldehyde-fixed NIH3T3 cells stained with AATF / Che-1 antibody. Center: same cells stained with Hoechst (DNA stain). Right: merge of images.

AATF / Che-1 antibody (mAb) tested by Western blot.

AATF / Che-1 antibody (mAb) tested by Western Blot.
AATF / Che-1 was detected in 20 µg of HeLa cell nuclear extract with AATC / Che-1 antibody at 2 µg / ml.
MW: 80 kDa


AATF/Che-1, or Apoptosis-antagonizing Transcription Factor is a key regulator of apoptosis through its interaction with MAP3K12 (DLK), as well as regulation of gene transcription and cell profileration through interaction with RNA polymerase II. AATF/Che-1 may promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) interaction with the growth inhibiting protein pRb. AATF / Che-1 binding to pRb may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. 

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AATF / Che-1 antibody (mAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting