AATF / Che-1 antibody (mAb)

RRID: AB_2793767
Clone: 1B2D8
Catalog No: 61789 Format: 100 µg $445 Buy
Catalog No: 61790 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Rat
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated

Application Notes


Validated Applications:
WB: 2 - 4 µg/ml
IF: 1:100 - 1:500

Published Applications:
WB


Ishigaki et. al., 2010 Cell Death Differ. 17: 774-786

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against peptide comprising amino acids 1-39 of mouse AATF (Apoptosis-antagonizing transcription factor) (NP_062790).

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. 

 

AATF / Che-1 antibody (mAb) tested by immunofluorescence.
Left: Formaldehyde-fixed NIH3T3 cells stained with AATF / Che-1 antibody. Center: same cells stained with Hoechst (DNA stain). Right: merge of images.

AATF / Che-1 antibody (mAb) tested by Western Blot.
AATF / Che-1 was detected in 20 µg of HeLa cell nuclear extract with AATC / Che-1 antibody at 2 µg / ml.
MW: 80 kDa

Background

AATF/Che-1, or Apoptosis-antagonizing Transcription Factor is a key regulator of apoptosis through its interaction with MAP3K12 (DLK), as well as regulation of gene transcription and cell profileration through interaction with RNA polymerase II. AATF/Che-1 may promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) interaction with the growth inhibiting protein pRb. AATF / Che-1 binding to pRb may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. 

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting