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Recombinant PARP-13 protein
| Aliases: Zinc finger CCCH type antiviral protein 1, ZC3HAV1, ARTD13, FLB6421, ZAP, ZC3H2, ZC3HDC2 |
| Catalog No: 82038 | Format: 20 µg | $505 | Add to Cart |
| Catalog No: 82638 | Format: 1 mg | $5,400 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
PARP-13 (Zinc finger CCCH-type antiviral protein 1), also known as ZC3HAV1, ARTD13, FLB6421, ZAP, ZC3H2 and ZC3HDC2. PARP-13 is an antiviral protein which inhibits the replication of viruses by recruiting cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3’-5’ exoribonuclease complex exosome to degrade the RNA body from the 3’-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5’-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1), moloney and murine leukemia virus (MoMLV) and xenotropic MuLV-related virus (XMRV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross River virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of RIGI signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs).
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant PARP-13 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_064504.2) with a C-terminal His and FLAG tag. The molecular weight of the protein is 103.95 kDa.
References
Human
Recombinant PARP-13 protein
10% SDS-PAGE Coomassie staining
MW: 103.95 kDa
Purity: ≥ 90%
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
