<< Back to SARS-CoV-2 Home Page
SARS-CoV-2 Overview & Product Data
Antibodies, Assays & Recombinant Proteins Developed by Active Motif
COVID-19 is one of the biggest public health issues in history and presents a unique challenge to the scientific research community – to develop reagents, diagnostics, and therapeutics more quickly than has ever been done.
Active Motif was one of the first companies to be involved in a key collaboration that led to the first cloning, expression, and characterization of recombinant human antibodies from patients who were infected with SARS-CoV-2, mounted an immune response, and recovered. Many of these recombinant antibodies are now available for commercial and research purposes. We also offer multiple recombinant SARS-CoV-2 proteins, including various formats of the SARS-CoV-2 Spike S1 protein, as well as many of the SARS-CoV-2 nonstructural proteins. We have additionally utilized these recombinant antibodies and proteins to create a SARS-CoV-2 Spike S1 protein ELISA.
Recombinant SARS-CoV-2 Antibodies
In partnership with our collaborators at Fudan University, Active Motif scientists successfully performed single-cell analysis of B cells from patients who recovered from COVID-19. Cells were cloned and their antibody genes were expressed, purified, and characterized to create recombinant human antibodies. Many of these have already been published and each is available in up to gram quantities.
SARS-CoV-2 Recombinant Antibody Reactivity with Viral Variants
|SARS-CoV-2 Spike Antibodies (Rec. Human IgG) EC50 [M]|
|SARS-CoV-2 Spike S1 RBD – Original virus, Cat. No. 81343||6.32E-11||5.46E-11||1.17E-10||8.11E-11||1.26E-10||2.31E-10||1.25E-10||1.40E-09|
|SARS-CoV-2 Spike S1 RBD – N501Y (UK)||4.80E-09||5.41E-11||1.04E-10||1.07E-10||1.30E-10||1.02E-09||8.48E-11||1.22E-09|
|SARS-CoV-2 Spike S1 RBD – K417N, E484K, N501Y (So. Africa)||3.96E-09||4.88E-11||1.34E-10||8.11E-11||7.51E-10||7.51E-10||9.91E-11||1.37E-09|
|SARS-CoV-2 Spike S1 RBD – K417T, E484K, N501Y (Brazil)||3.23E-10||2.83E-10||2.89E-10||2.36E-08||8.23E-09|
|SARS-CoV-2 Spike Trimer – Original virus||3.62E-11||4.56E-11||1.47E-10||1.02E-10||6.60E-11||2.06E-09||2.69E-10||3.72E-09|
|SARS-CoV-2 Spike S1 protein, a.a. 16-685||1.35E-08||5.53E-11||1.16E-10||2.58E-10||1.42E-10||6.52E-09||2.41E-10||3.08E-09|
|SARS-CoV-2 Spike Trimer – B.1.1.7 (UK)||1.53E-08||6.06E-11||3.88E-10||1.64E-09||2.19E-10||3.40E-08||8.83E-10||5.96E-09|
|SARS-Cov-2 Spike Trimer – B.1.351 (So. Africa)||3.76E-10||9.42E-10||5.83E-10||2.30E-08|
SARS-CoV-2 Recombinant Antibodies - Human Ig
AbFlex® SARS-CoV-2 Recombinant Antibodies - Mouse Ig
|SARS-CoV-2 Spike S1 Protein Binding|
|Product Name||Cat. No.||Applications||RBD aa319-541,
Cat. No. 81343
Cat. No. 81344
|SARS-CoV-2 Spike Antibody (AM001414)||91375 / 91376||ELISA, Neu||Yes||Yes||More Info|
|SARS-CoV-2 Spike Antibody (AM002414)||91363 / 91364||ELISA, Neu||Yes||Yes||More Info|
|SARS-CoV-2 Spike Antibody (AM004414)||91373 / 91374||ELISA, WB||No||Yes||More Info|
|SARS-CoV-2 Spike Antibody (AM009105)||91365 / 91366||ELISA||Yes||Yes||More Info|
|SARS-CoV-2 Spike Antibody (AM038105)||91371 / 91372||ELISA||Yes||Yes||More Info|
|SARS-CoV-2 Spike Antibody (AM043105)||91367 / 91368||ELISA||Yes||Yes||More Info|
We are also looking for partners and collaborators to continue the development of these recombinant antibodies for diagnostic and/or therapeutic purposes. Contact us for more information.
Recombinant SARS-CoV-2 Spike S1 Protein ELISA
As the SARS-CoV-2 Spike S1 protein is displayed on the surface of the viral particles, antibodies that bind very specifically and with high affinity to the S1 protein can be used in immunological assays like ELISAs to detect the presence of the virus in biological samples. Each S protein contains two subunits, S1 and S2, with the S1 subunit mainly comprised of the receptor-binding domain (RBD). It is the RBD which is responsible for recognizing the cell surface receptor ACE2.
The SARS-CoV-2 Spike S1 Protein ELISA Kit is designed for the detection of SARS-CoV-2 Spike S1 RBD in a convenient 96-stripwell plate, enabling you to simultaneously process up to 96 samples in a single experiment. For added convenience and a more quantitative interpretation of the results, the kit also includes recombinant Spike S1 RBD which enables you to build a reference standard curve.
SARS-CoV-2 Spike S1 Protein ELISA Kit Highlights:
- Pre-coated wells for faster time-to-result
- Designed for high-throughput use in a 96-well format
- Validated to detect Spike S1 protein in human saliva and plasma samples
SARS-CoV-2 Spike S1 Protein ELISA Overview: The kit is based on the sandwich enzyme immunoassay technique, using pre-coated well strips with recombinant SARS-CoV-2 Spike S1 antibody. Both recombinant SARS-CoV-2 Spike S1 protein standards and your samples are added to the pre-coated wells. Spike S1 present in the sample(s) is captured by the antibody. A biotinylated SARS-CoV-2 detection antibody is then added, producing the antibody-antigen-antibody “sandwich” immunocomplex. Streptavidin-horseradish peroxidase (HRP) conjugate is then added, followed by the developing solution to enable a sensitive colorimetric readout that is easily quantified by spectrophotometry.
SARS-CoV-2 Spike S1 Protein ELISA Kit – validated for biological samples including saliva and plasma.
Recombinant SARS-CoV-2 Spike S1 protein generates reliable, consistent standard curves.
Recombinant SARS-CoV-2 Proteins
Active Motif manufactures our own high-quality recombinant proteins, including SARS-CoV-2 spike S1 proteins, and a number of SARS-CoV-2 nonstructural proteins (NSPs). Each of these proteins is available in up to 1 mg quantities that are in stock and ready to ship. Contact us to inquire about bulk orders.