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CENP-B antibody (pAb)

Catalog No: 61287 Format: 100 µl $425 Buy Now
Catalog No: 61288 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:80 kDa


Western Blot Validated Immunoprecipitation Validated

for CENP-B antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for CENP-B antibody (pAb)Immunogen

This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.

for CENP-B antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CENP-B antibody (pAb)References

CENP-B antibody (pAb) tested by immunoprecipitation.

CENP-B antibody (pAb) tested by Immunoprecipitation.
10 µl of CENP-B antibody was used to immunoprecipitate CENP-B from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.

CENP-B antibody (pAb) tested by Western blot.

CENP-B antibody (pAb) tested by Western blot.
CENP-B detection by Western blot. The analysis was performed using 30 µg HeLa nuclear cell extract and CENP-B at a 1:500 dilution.

for CENP-B antibody (pAb)Background

CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the α-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.

for CENP-B antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CENP-B antibody.

for CENP-B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CENP-B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

CENP-B antibody (pAb)

Data Thumbnails

CENP-B antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of CENP-B pAb.
(Click image to enlarge and see details.)

CENP-B antibody (pAb) tested by Western blot.

Western blot of CENP-B pAb.
(Click image to enlarge and see details.)