Recombinant GOT1 (AST1) protein

Aliases: glutamic-oxaloacetic transaminase 1
Catalog No: 81297 Format: 50 µg $450 Buy
Catalog No: 81997 Format: 1 mg $3,000 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

GOT1 (Glutamic-Oxaloacetic Transaminase 1), also known as AST1, is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT1 catalyzes the reversible conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate in amino acid metabolism and is found in tissues of liver, heart, skeletal muscle, brain, and red blood cells. The enzyme is commonly used as a clinical marker since the serum level of GOT1 indicates the severity of damaged liver and heart tissue. GOT1 expression correlates with tumor growth due to use of the glutamine by various cancer cells. GOT1 is a homodimer where each monomer comprises a small and large domain. Two identical PLP binding sites are located at the dimeric interface between the small and large domains of each monomer. Residue K259 in the catalytic pocket is essential for the formation of the PLP-K259 aldimine and the R387/R293 substrate binding motif.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Full length human GOT1 / AST1 protein was expressed in E. coli cells (accession number NP_002070.1) with a C-terminal 6xHis Tag. The molecular weight of the protein is 47.3 kDa.

 
Recombinant GOT1 / AST1 protein gel

Recombinant GOT1 / AST1 protein gel
10% SDS-PAGE with Coomassie blue staining

MW: 47.3 kDa

Purity: >95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of arrival.