Recombinant PHF2 protein

Aliases: PHD finger protein 2
Catalog No: 82174 Format: 20 µg $600 Add to Cart
Catalog No: 82774 Format: 1 mg $6,000 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant PHF2 protein gel

Recombinant PHF2 protein gel
4-20% SDS-PAGE gel
MW: 118.53 kDa
Purity: >85%

Background

Recombinant human PHF2 (PHD finger protein 2), also known by its gene symbol PHF2 and alternative names including JHDM1E (Jumonji C domain-containing histone demethylase 1E), KDM7C (lysine-specific demethylase 7C), CENP-35 (centromere protein 35), and GRC5, is a flavin adenine dinucleotide (FAD)-binding protein that functions as a histone demethylase specifically targeting dimethylated Lys-9 of histone H3 (H3K9me2) to promote transcription activation of target genes. This recombinant form, typically produced in expression systems such as E. coli, retains the key biochemical properties of the native protein, including its JmjC domain that confers demethylase activity and its PHDtype zinc finger that mediates binding to methylated histones. PHF2 plays critical roles in diverse biological processes including adipocyte differentiation where it promotes expression of major transcription factors, liver development as a coactivator of HNF4A, and activation of toll-like receptor 4-target inflammatory genes in macrophages.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting