Recombinant SIRT1 (193-741) protein

Aliases: Sirtuin 1, SIRT-1, SIR2-Like Protein, SIR2L1, SIR2alpha
Catalog No: 31533 Format: 100 µg $420 Buy
Catalog No: 31933 Format: 1 mg $2,650 Buy

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Expressed In: E. coli Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD+-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

Application Notes

Recombinant SIRT1 protein is useful for histone deacetylase assays and the study of DNA Repair.

Active Motif has a collection of small-molecule epigenetic modulators that target SIRTs - click here to view the list.

Protein Details

Recombinant SIRT1 (193-741) protein that includes amino acids 193 - 741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa. The purity of the protein is > 60% by SDS-PAGE.

 

Recombinant SIRT1 (193-741)12.5% SDS-PAGE with Coomassie Blue staining.
MW: 101.5 kDa
Purity: >60%

HTRF assay for SIRT1 (193-741) activity
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37 °C. Anti-H3K9me0 antibody was used to detect reaction products.

HTRF assay for SIRT1 (193-741) activity
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.